Super active Ubrinuclease™ pan endonuclease, purified in compliance with regulatory standards, exhibits broad nuclease activity for all types of nucleic acids.
The Ubrinuclease™ pan endonuclease effectively digests any form of DNA and RNA (double-stranded, single-stranded, linear, circular, native, or denatured) into 3-5 bases 5'-monophosphate oligonucleotides under a wide range of conditions.
High purity & high pan endonuclease activity
Free of Animal derived materials, fully compliant with regulatory standards
Ideal for removing DNA and RNA in the production of biologics
Tag-free or His/ Strep-Tag
Activated FDA DMF on file #29414
| Product Name | Quality Grade | Catalog Number | Size | Price |
|---|---|---|---|---|
| Ubrinucelase™ Pan endonuclease | GMP grade | UbNuC-G100 | 100KU | Please Inquire |
| UbNuC-G500 | 500KU | |||
| UbNuC-G1000 | 1MU |
GMP grade pan endonuclease that works with any DNA and RNA.
Request a Quote
Ubrinucelase™ pan endonuclease (GMP grade) can be used for discovery research, and as well as GMP manufacturing of cell and gene therapies, vaccines, and other biologics. The GMP-grade Ubrinucelase™ pan endonuclease products are manufactured in compliance with the highest regulatory standards.
Below are a few examples of applications that the pan endonuclease can used:
Protein production to reduce viscosity, increasing protein yield.
Viral vector manufacturing to remove nucleic acids on the surface of viral particles, decreasing unwanted residual DNA.
Sample preparation, including ELISA, chromatography, 2D electrophoresis, and blotting, improving resolution and recovery rates.
Vaccine, polysaccharides, and other biologics to remove residual nucleic acids.
Preventing PBMC clumping in cell therapy and vaccine research
| Source | S. marcescens |
|---|---|
| Expression Host | E. coli |
| Molecular Weight | ～30KD |
| Isoelectric Point | 6.39/6.85/7.1 |
| Appearance | Clear |
| PH | 7.0-9.0 |
| Tag | Tag-free/ His-tag/ Strep-tag |
| Purity | ≥99%（SDS-PAGE/SEC-HPLC） |
| Specific Activity (calc. on protein) | ≥ 1.0×106 U/mg |
| Enzyme Activity | ≥ 250 U/μL |
| Activity Definition | One unit (U) is defined as the amount of enzyme required to change the absorbance at 260 nm by 1.0 (equivalent to complete digestion of 37 μg of salmon sperm DNA into oligonucleotides) in a 2.625 mL reaction system at 37°C, pH 8.0, within 30 minutes. |
| Sterility | Negative |
| Mycoplasma | Negative |
| Microbial Count | ＜1cfu/mL |
| Endotoxin | ＜0.25EU/KU |
| HCP | ＜10μg/mg |
| Heavy Metal Residuals | ≤10ppm |
| Protease Activity | Negative |
| Buffer | 20 mM Tris-HCl pH8.0，2 mM MgCl2，20 mM NaCl，50% glycerin |
| Optimal Temperature & pH | 37℃，8.0 |
Shipping on ice; stored at -20°C for long-term storage, with a shelf life of 2 years.
SDS-PAGE and SEC-HPLC analysis confirm Ubrinucelase™ Pan Nuclease has a purity above 99%.
Pure Ubrinuclease™ Pan Endonuclease analyzed by SDS-PAGE (left) and SEC-HPLC (right).
Ubrinuclease™ shows superior endonuclease activity compared to competitor products and is more tolerant of varying salt concentrations (0–0.5M NaCl, 30 min). This adaptability improves its efficiency in diverse biologics purification processes.
Notes for endonuclease:
EDTA, urea, SDS, and guanidine hydrochloride can inhibit or inactivate the enzyme.
Repeated freeze-thaw cycles affect the enzymatic activity. Small aliquots are recommended for storage; do not store at -80°C.
For high-salt, acidic, or basic solutions, or those with high concentrations of detergents or denaturants, increase enzyme dosage or extend incubation time to maximize enzyme activity.
Ubrinuclease™ Pan Endonuclease is used to digest unwanted DNA and RNA in biologics and cell and gene therapy manufacturing workflows. Its broad substrate activity supports nucleic-acid removal during processes such as viral vector manufacturing and biologics purification, helping control residual nucleic-acid impurities.
Ubrinuclease™ is designed to digest double-stranded and single-stranded DNA and RNA, including linear, circular, native, and denatured nucleic acids. Digestion produces short oligonucleotides of approximately 3–5 bases with 5′-monophosphate ends.
Ubrinuclease™ demonstrates high nuclease activity and tolerance across a range of salt concentrations, including 0–0.5 M NaCl under tested conditions. Comparative performance can vary with enzyme concentration, substrate, buffer composition, incubation time, and other process conditions, so application-specific side-by-side testing is recommended.
Yes. uBriGene offers GMP-grade Ubrinuclease™ Pan Endonuclease manufactured under an active FDA Drug Master File (DMF) #29414. The enzyme is animal-component-free and is intended for nucleic-acid removal in GMP manufacturing workflows for cell and gene therapies, vaccines, and other biologics, subject to the requirements of the specific manufacturing process.
Ubrinuclease™ can be used during AAV manufacturing, lentiviral vector (LVV) production, and other viral-vector manufacturing workflows to digest residual host-cell and process-related DNA and RNA. Nuclease treatment can help reduce nucleic-acid impurities before downstream purification, with residual levels evaluated using appropriate analytical methods such as qPCR.
Ubrinuclease™ shows optimal activity at approximately 37°C and pH 8.0 under tested conditions and maintains activity across NaCl concentrations of 0–0.5 M. Actual performance depends on substrate, enzyme concentration, ionic strength, incubation time, and enzyme-to-substrate ratio, so process conditions should be optimized for the intended application.
Ubrinuclease™ should be stored according to the product instructions, with small aliquots recommended to minimize repeated freeze-thaw exposure. Repeated freeze-thaw cycles can reduce enzymatic activity, and the product information specifies that the enzyme should not be stored at −80°C. When stored at −20°C according to product recommendations, Ubrinuclease™ has a 2-year shelf life.
Important factors include DNA and RNA substrate coverage, nuclease activity, salt tolerance, process compatibility, impurity-removal requirements, raw-material quality, and GMP documentation. Ubrinuclease™ provides broad DNA/RNA substrate activity, activity across 0–0.5 M NaCl under tested conditions, and a GMP-grade option supported by FDA DMF #29414.
Digest on any DNA and RNA
Manufactured according to GMP requirement
