Third-generation GMP lentivirus packaging plasmids, manufactured in compliance with the highest regulatory standards, featuring active DMF to streamline the LVV IND review process.
Accelerate your GMP Lentivirus Production with uBriGene's ready-to-use lentivirus plasmids, including pGagPol, pRev, and pVSV-G.
uBriGene has supported clients in achieving CAR-T IND clearance using lentiviral vectors produced with our GMP lentivirus packaging plasmids.
Manufactured and quality released under stringent GMP and regulatory standards.
Available in both R&D and GMP-grade lentivirus plasmids.
Royalty-free for use with uBriGene's GMP Lentivirus Production Services.
Backed by an active FDA DMF #27777 to support regulatory submissions.
| Product Name | Catalog Number | Description | Grade | E. Coli Selection | Price |
|---|---|---|---|---|---|
| Hex-VSVG | 1302HEX-VSVG | pVSV-G envelope packaging plasmid for pseudo lentivirus | ☑Research ☑GMP | KanR | |
| Hex-MDL | 1302HEX-MDL | pGag-Pol packaging plasmid for lentivirus | ☑Research ☑GMP | KanR | 50% off GMP lentivirus plasmids |
| Hex-Rev | 1302HEX-REV | pRev packaging plasmid for lentivirus | ☑Research ☑GMP | KanR |
Ready for GMP lentivirus production
GMP pGagPol, pRev, pVSV-G plasmids
Active FDA DMF #27777
Sit aliquam elit vitae bibendum lectus blandit dictum. Nisi porta elementum sed diam eleifend pellentesque. Quis aenean quam.
Request a Quote
Automated closed-system technology for manufacturing ensures sterility with functionally closed disposables, minimizes operator errors through pre-programmed steps, and enhances consistency by reducing variability. It also reduces technician hands-on time and lowers production costs.
Extensive plasmid production experience
100+ GMP plasmid projects annually
Our third-generation lentivirus plasmids are used in our lentivirus GMP manufacturing. High quality and high yield of lentiviruses are produced with our proprietary LVV suspension production cell line, 293TH. Transduction titer is measured after transducing Jurkat cells, ranging from 1-5 E7 TU/mL, depending on the target genes.
uBriGene’s lentivirus packaging plasmids were used for lentiviral vector production with uBriGene’s proprietary LVV suspension cell bank, 293TH. Transduction titer was measured using flow cytometry after transducing Jurkat cells.
As a world leading GMP plasmid DNA manufacturer, we provide exceptional QA and QC support, with most plasmid QC release assays performed in-house.
Our off-the-shelf lentivirus packaging plasmids are manufactured and released in strict compliance with the highest regulatory standards. A certificate of analysis will be provided with each of the GMP grade lentiviral packaging plasmids.
| Test Item | Method | |
|---|---|---|
| Appearance | Visual inspection | |
| pH | pH measurement | |
| Concentration | UV, Spectrophotometry | |
| Identity | Restriction analysis | Gel electrophoresis |
| Target sequence analysis* | Sanger sequencing | |
| Purity | A260/A280 | UV, Spectrophotometry |
| Supercoiled plasmid ratio | HPLC | |
| Residuals | Residual E. Coli DNA | qPCR |
| Residual E. Coli RNA | Gel electrophoresis/HPLC | |
| Residual E. Coli protein | ELISA | |
| Safety | Endotoxin | Gel-clot assay |
| Sterility | Membrane filtration method | |
Explore uBriGene’s robust GMP plasmid manufacturing process and testing capabilities.
Unveil the current manufacturing approaches for LVV and its deployment in cell therapies
Learn about CAR-T production process & regulatory guidelines.
Learn about uBriGene’s RNA and LNP manufacturing capacities and testing.
GMP lentiviral packaging plasmids should be manufactured and controlled under appropriate quality systems to ensure identity, sequence integrity, purity, and consistent quality for their intended use in LVV manufacturing. Key controls may include plasmid identity and sequence confirmation, purity, supercoiled content, residual host-cell impurities, endotoxin, sterility, and appropriate release documentation. uBriGene’s GMP packaging plasmids include pGagPol, pRev, and pVSV-G and are supplied with quality documentation, including a Certificate of Analysis.
uBriGene offers GMP-grade third-generation lentiviral packaging plasmids, including pGagPol, pRev, and pVSV-G. These plasmids are supplied as quality-released materials for use in lentiviral vector manufacturing.
uBriGene’s GMP lentiviral packaging plasmids are supported by FDA Drug Master File (DMF) #27777. A DMF can provide CMC information that a sponsor may reference in an appropriate regulatory submission, reducing the need to reproduce certain manufacturing and quality information. The DMF itself does not guarantee faster FDA review or IND clearance.
Yes. uBriGene’s GMP-grade pGagPol, pRev, and pVSV-G packaging plasmids can support LVV programs from preclinical development through clinical manufacturing, subject to the material, process, quality, and regulatory requirements established for the intended stage. Using qualified GMP plasmids can help maintain material consistency as an LVV program progresses toward clinical manufacturing.
GMP-grade packaging plasmids provide controlled, quality-released production materials that support consistent LVV manufacturing and regulatory documentation. Using qualified plasmids can help reduce variability in critical starting materials and provide defined quality attributes, release testing, and traceability. uBriGene’s GMP pGagPol, pRev, and pVSV-G plasmids are supported by FDA DMF #27777 and can be used within its scalable LVV manufacturing platform.
Yes. uBriGene’s GMP pGagPol, pRev, and pVSV-G packaging plasmids are used within its scalable LVV manufacturing platform. The platform supports suspension-cell LVV production using 293TH cells, with manufacturing scale and process conditions established according to the vector construct, target output, and development stage. Reported LVV production titers can vary with the target gene and manufacturing conditions.
uBriGene’s GMP lentiviral packaging plasmids undergo QC testing appropriate to their intended use and manufacturing requirements. Testing can include appearance, concentration, identity, target-sequence analysis, purity, supercoiled plasmid ratio, residual host-cell DNA/RNA/protein, endotoxin, and sterility. Quality documentation, including a Certificate of Analysis, is provided for released plasmids.
Yes. uBriGene’s GMP lentiviral packaging plasmids are available royalty-free for applicable lentiviral vector manufacturing programs.
uBriGene’s off-the-shelf GMP lentiviral packaging plasmids are typically available for shipment within 1–2 weeks after order confirmation, subject to product availability and order requirements.
In a third-generation lentiviral packaging system, pGagPol provides structural and enzymatic proteins required for vector production, pRev supports export of viral RNA from the producer cell, and pVSV-G provides the envelope protein used to pseudotype the lentiviral vector. uBriGene offers GMP-grade versions of all three packaging plasmids.
Yes, sponsor-provided lentiviral packaging plasmids can be evaluated for compatibility with uBriGene’s LVV manufacturing platform. Additional process development, testing, or qualification may be required depending on the plasmid design, quality attributes, and manufacturing strategy. uBriGene also provides GMP pGagPol, pRev, and pVSV-G packaging plasmids for programs requiring qualified plasmid supply.
Extensive expertise with a track record of successfully releasing over 60 GMP batches of AAV.
GMP grade pGagPol, pRev, pVSV-G
Activated FDA DMF on file #27777
Proven - LVV produced using the Lentivirus packaging plasmids received IND clearance
Sit aliquam elit vitae bibendum lectus blandit dictum. Nisi porta elementum sed diam eleifend pellentesque. Quis aenean quam.
Request a QuoteAsk the plasmid DNA expert! With over 300 GMP batches in our track record and our cost-effective platform technology, we can help accelerate your clinical programs.
