Ready-to-use, non-integrating reprogramming products featuring RNA-LNP cocktails for efficient generation of induced pluripotent stem cells (iPSCs).
The iPSC Reprogramming Cocktail is a simple LNP composed of RNAs for five reprogramming factors. It efficiently reprograms human skin fibroblasts, PBMCs, and other somatic cells into iPSCs with low toxicity compared to other RNA kits.
Reprogramming with RNA, no risk of gene integration
Convenient, Ready-to-use LNP mix, can be directly added to cells, eliminating the need for transfection reagents or viral manipulation
Low cytotoxicity, high iPSC reprogramming efficiency
System flexibility: suitable for feeder or feeder-free conditions
The various Rep/Cap plasmids and helper plasmids have been tested for high quality and high yield in GMP AAV production.
We have assisted our clients in obtaining IND clearance with AAV produced using these AAV helper plasmids.
Our RNA-LNP iPSC reprogramming cocktail delivers high-efficiency results in a simple, ready-to-use format — just add directly to cells.
| Catalog Number | Description | Product Size | Product Grade | Price (Research Grade) |
|---|---|---|---|---|
| PSC-RPM-mRNA-LNP-FB-S | An efficient iPSC reprogramming mRNA-LNP cocktail for fibroblasts, can be directly added to cells | Trial size 2x60uL (1 x iPSC reprogramming) | ☑Research ☐GMP | $450 |
| iPSC-RPM-mRNA-LNP-FB | Standard size 8x60uL (5 x iPSC reprogramming) | ☑Research ☑GMP | $1,475 |
| Catalog Number | Description | Product Size | Product Grade | Price (Research Grade) |
|---|---|---|---|---|
| iPSC-RPM-RNA-LNP-V2-S | An efficient iPSC reprogramming RNA-LNP kit for PBMCs, fibroblasts, difficult-to-reprogram, and disease-derived cells, can be directly added to cells | 1x80uL RNA-LNP cocktail, 1x30uL enhancer B (2 x iPSC reprogramming) | ☑Research ☐GMP | $995 |
| iPSC-RPM-RNA-LNP-V2 | 3x80uL RNA-LNP cocktail, 1 x enhancer B (80 uL) (6 x iPSC reprogramming reactions) | ☑Research ☑GMP | $2,680 | |
| LNP-ENHANCER-B | Enhancer B | 80uL / vial | ☑Research ☑GMP | $750 |
| PBMC-COMP-MEDIA-01 | PBMC-Comp-Media-01 | 50mL / vial | ☑Research ☐GMP | $450 |
Note: Research use only. Licensing is required for commercial use. View the product's LULL.
PBMC Kit (Cat# iPSC-RPM-RNA-LNP-V2): This kit can efficiently reprogram PBMCs, fibroblasts, and hard-to-reprogram cells.
| Reprogramming Cell Types | PBMC & difficult-to-reprogram cell reprogramming kit (cat# iPSC-RPM-RNA-LNP-V2) |
|---|---|
| Fibroblasts from young donors | √ |
| Fibroblasts from aged donors | √ |
| Disease-derived fibroblasts | √ |
| PBMCs | √ |
| Cells from aged donors | √ |
| Patient-derived cells | √ |
GMP grade iPSC reprogramming RNA-LNP cocktail
Sit aliquam elit vitae bibendum lectus blandit dictum. Nisi porta elementum sed diam eleifend pellentesque. Quis aenean quam.
Request a Quote
Optional caption section: This caption text can be used under any image or content block as-needed. Use cases are figure legends, citations, image descriptions
Extensive iPSC generation and banking experience with access to essential raw materials, including iPSC reprogramming mRNA-LNP mix and CRISPR gene editing tools.
Extensive plasmid production experience
100+ GMP plasmid projects annually
Somatic cells (fibroblasts, PBMCs, or other cell types) are seeded onto feeder or feeder-free plates. RNA-LNP cocktails are directly added to the cell culture at a frequency specific to each cell type. After treatment with the iPSC Reprogramming Cocktail, cells are maintained in reprogramming medium throughout the induction phase until iPSC colonies emerge. Typically, by Day 15, the colonies are sufficiently large for selection and expansion.
Fully characterized iPSC banks and lines, generated using our RNA-LNP reprogramming kit, are available to accelerate your cell therapy research and clinical applications.
Fig. 1. iPSC reprogramming diagram using uBriGene’s reprogramming mRNA-LNP cocktail mix.
Fig. 1. iPSC reprogramming diagram for PBMCs using the Ubri-iPSC reprogramming RNA-LNP kit.
By day 4, cell morphology changed significantly. Deformable cells increased by day 6, and aggregated cells appeared by day 8 of reprogramming. iPSC-like cells emerged on day 11, and typical iPSC colonies formed by day 13.
Fig. 2. Cell morphologies following treatment with Ubri-iPSC Reprogramming RNA-LNP. (A) Reprogramming of fibroblasts; (B) Reprogramming of PBMCs.
Human skin fibroblasts were reprogrammed into iPSCs using uBriGene’s iPSC Reprogramming mRNA-LNP Cocktail, and the colonies were identified with AP staining on day 18. The results showed that iPSC colonies were positive for AP staining, with reprogramming efficiency reaching as high as 10%.
Fig. 3. iPSC identification using Alkaline phosphatase staining after the iPSC reprogramming process with mRNA-LNP cocktail.
iPSCs generated using our proprietary RNA-LNP reprogramming kit exhibit robust expression of stemness and pluripotency. Following reprogramming, the cells are rigorously characterized to confirm genomic stability and absence of residual RNA.
Fully established research-grade iPSC lines and GMP-compliant iPSC banks are available to support your regenerative and allogeneic therapy programs.
| Test Item | Assay |
|---|---|
| Sterility | Immersion Sterility tests (B/F) |
| Mycoplasma Testing | Culture and cell indication assay |
| Endotoxin | Kinetic chromogenic LAL |
| Cell Viability | Trypan Blue |
| Pluripotency Characteristics | Flow cytometry |
| Embryoid body formation | |
| Genetic stability | STR genotyping |
| Karyotype analysis | |
| Adventitious Viruses | Transmission electron microscopy (TEM) |
| In vitro assay for adventitious virus contaminants | |
| Fluorescent Product Enhanced Reverse Transcriptase (FPERT) method |
These simplified iPSC reprogramming procedures outline the basic steps to generate iPSCs from fibroblasts or PBMCs using our reprogramming RNA-LNP cocktails.
iPSC Reprogramming Protocol Using RNA-LNP Kit
Step 1: Culture fibroblasts, PBMCs, or other cell types with cell-type specific culture medium.
Step 2: Treat cells with the RNA-LNP cocktail every other day for a total of four treatments. Add Serum-Free Enhancer B to the media. BioLaminin 521 is recommended for plate coating.
Step 3: Change the media daily using ReproTeSR and monitor for iPSC colony formation.
Step 4: Pick and expand iPSC colonies between Days 16–20. Confirm iPSC formation by AP staining and validate pluripotency with markers such as SSEA4, TRA-1-81, SOX2, and NANOG.
Learn how to accelerate the development of iPSC-derived cell therapies.
Unlock safer regenerative therapies with Genome-Safe iPSC RPM for clinical success.
Redefining RNA Therapeutics: GMP RNA-LNP Manufacturing You Can Trust
Explore RNA drug development fundamentals and the vital role of IVT in manufacturing.
uBriGene’s iPSC Reprogramming mRNA-LNP cocktail is a ready-to-use lipid nanoparticle formulation containing mRNAs for five reprogramming factors. It is designed to efficiently reprogram somatic cells, like human fibroblasts, into iPSCs without altering the cell genome.
The mRNA-LNP cocktail delivers reprogramming mRNAs into somatic cells, triggering them to convert into induced pluripotent stem cells (iPSCs). This non-integrating method reduces the risk of permanent changes to the genome.
mRNA-LNP reprogramming cocktail avoids the risk of gene integration, offers high efficiency, and has low cytotoxicity. It also simplifies the process since no transfection reagents or viral manipulation are required.
The reprogramming process usually takes about 15–18 days. iPSC colonies typically emerge around day 13, and are large enough for selection and expansion by day 15.
Yes, iPSCs generated using uBriGene’s mRNA-LNP cocktail are pluripotent. They express key pluripotency markers such as SSEA4, TRA-1-81, Sox2, and Nanog.
The iPSC reprogramming mRNA-LNP cocktail has been optimized for use with human fibroblasts and PBMC, but can be tested on other somatic cells as well, depending on the research needs.
iPSC cell banks are tested for the expression of undifferentiated cell markers (SSEA4, TRA-1-81, Sox2, and Nanog) via flow cytometry to ensure high-quality iPSC generation.
It is recommended to split the cells immediately after completing the eight RNA-LNP treatments. To prevent overcrowding, you can use two dilutions, 1:12 and 1:24. If the cells are split several days after the RNA-LNP treatment, the resulting colonies may originate from the same reprogrammed cells.
Extensive expertise with a track record of successfully releasing over 60 GMP batches of AAV.
GMP grade iPSC reprogramming mRNA & mRNA-LNP cocktail
iPSC Cell Banks for clinical use
Sit aliquam elit vitae bibendum lectus blandit dictum. Nisi porta elementum sed diam eleifend pellentesque. Quis aenean quam.
Request a QuoteOptional caption section: This caption text can be used under any image or content block as-needed. Use cases are figure legends, citations, image descriptions
Ask the plasmid DNA expert! With over 300 GMP batches in our track record and our cost-effective platform technology, we can help accelerate your clinical programs.
