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An illustration of animal cells

AAV Packaging

AAV (Adeno-associated virus) is a parvoviridae virus belonging to the genus Dependoparvovirus. It has a simple structure which is replication-defective, non-enveloped viruses with single stranded DNA. AAV exhibits high tissue specificity, good transmissibility, low immunogenicity, high safety and stability compared to other viral vectors. These characteristics make recombinant adeno-associated viruses (rAAV) one of the promising viral vectors for basic research and gene therapy with significant market potential. 

uBriGene has established an advanced viral packaging platform with top-quality AAV packaging process. Rigorous quality control and comprehensive process quality management ensure the provision of high-titer and high-purity off-the-shelf and customizable AAV to our clients.

Packaging Processing Flow

Processing flow for AAV packaging service

uBriGene also offers high-quality custom AAV packaging services tailored to your experimental needs. With the support of our well-established and reliable AAV packaging platform and cutting-edge suspension cell packaging processes, the AAV viruses we prepare are characterized by low impurities, high purity (Full AAV can reach 95%), high titers (above 1E+13 GC/ml), and an endotoxin level below 2 EU/mg, surpassing the highest industry standards. 

Scope of Services

Service Type
Packaging Specifications
Delivery Time and Quotation
Gene Overexpression AAV
1*10E12vg, 1*10E13vg, 1*10E14vg, 1*10E15vg, 1*10E16vg, Additional specifications
shRNA Interference AAV
1*10E12vg, 1*10E13vg, 1*10E14vg, 1*10E15vg, 1*10E16vg, Additional specifications
CRISPR/gRNA AAV
1*10E12vg, 1*10E13vg, 1*10E14vg, 1*10E15vg, 1*10E16vg, Additional specifications

uBriGene can provide both small-scale preparations (in vivo/ vitro) as well as large-scale cGMP production for clinical applications. Our services cater to various needs in the fields of scientific research and clinical studies on gene therapy. Our company currently possesses plasmids with dozens of AAV serotypes, allowing for the preparation of various viruses with different capsids. With our own patented helper plasmids and high-quality AAV viral vectors, we stand out among viral packaging services in the industry. Our supplementary services, including AAV CRISPR and AAV vector QC test upon request, further enhance our products and services. Furthermore, we can select AAV serotypes with stronger tissue specificity based on your specific requirements. These can be combined with numerous promoter elements to better meet your tissue-specific needs. 

Different AAV Tissue-Specific Serotypes 

Tissue
Serotype
Heart
AAV9, AAV6, AAV1
Nerves
PHP.EB, AAV9, AAV-retro, AAV1, AAV4, AAV8, AAV5
Liver
AAV9, AAV8, AAV7, AAV2, AAV3
Lung
AAV5, AAV9, AAV6, AAV1, AAV2
Muscle
AAV9, AAV8, AAV7, AAV2, AAV6
Kidney
AAV9, AAV2, AAV5, AAV1
Adipose
AAVRec2, AAV1
Intestine
AAV8, AAV2, AAV5, AAV1
Pancreas
AAV9, AAV8
Eyes
AAV2, AAV1, AAV5

Common QC Item: Titer assay (ddPCR) 

AAV packaging Quality control item - Titer assay

Common QC Item: Purity assay (AUC) 

AAV packaging Quality control item - Purity assay

More QC Items 

Inspection Items
Inspection Items
Inspection Methods
Identification
Capsid protein identification
SDS-PAGE
Content
Virus physical titer
ELISA
Content
Virus genome titer
qPCR/ddPCR
Impurities
Host DNA residue
qPCR
Impurities
Plasmid DNA residue
qPCR
Impurities
Host cell protein residue
ELISA
Safety
Bacterial endotoxin
Gel-clot
Purity
Capsid protein purity
SDS-PAGE
Purity
Empty capsid rate
AUC
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