AAV Products Highlights
uBriGene has been striving for excellence in the field of plasmid and AAV production for years. With a professional technical team, solid theoretical foundation, and extensive production experience, we have high-quality off-the-shelf AAV products including genes for fluorescent proteins, optogenetics, chemical genetics, calcium indicators, neurotransmitter fluorescent probes, Cre & Flp recombinase, as well as autophagy and Pcsk9 atherosclerosis modeling tools. We offer more than 10 AAV serotypes, more than 40 promoters (broad-spectrum & tissue-specific), as well as constitutive & inducible expression methods to meet different experimental needs.
uBriGene is one of the few CDMO companies experienced in the industrialized production of AAV. We have independently developed AAV-packaging cells, notably the self-innovated, patented 293XS suspension culture cell line.
As the most critical raw materials for AAV production, large-scale production of plasmids is crucial. The whole process of uBriGene plasmid production and fermentation is serum, animal-ingredients, and antibiotics free. The purification steps adopt an innovative and efficient two-step column chromatography process, and plasmid yield can reach 1g/L. We also provide an AAV purification platform for multiple serotypes, with high yield and low empty shell rate, and can be linearly scaled up to 200-500L.
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Why is a three-tiered cell bank required?According to regulations, a complete culture cell bank must be established before GMP-grade plasmid production, including the primary cell bank, the master cell bank and the working cell bank.
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What is the storage temperature for plasmids? How long can they be stored?According to BriGene’s many years of GMP plasmid production experience, the storage temperature of plasmids is -20°C, and the long-term storage stability data can be traced back for more than half a year.
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Which strain is used to produce plasmids?uBriGene uses DH5α or Stbl3 for plasmid production, depending on the customer's choice.
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Why are plasmids modified for kanamycin resistance?Regulations require that the vectors used in gene therapy drugs must not introduce resistance to penicillin.
Other Products
Product | Gene of Interest | Scale |
|---|---|---|
Hex-VSVG | VSV-G | >10mg |
Hex-Rev | Rev | >10mg |
Hex-MDL | HIV-1 GAG/POL | >10mg |
Related Services
IIT non-registered clinical study / investigational new drug (IND) application / gene therapy commercialization